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Image Search Results
Journal: STAR Protocols
Article Title: Protocol for the genome-wide identification of intrinsic transcription factor binding motifs by mammalian-optimized pull-down sequencing
doi: 10.1016/j.xpro.2026.104513
Figure Lengend Snippet: Expression and purification of recombinant His-MBP-FOXP3(ΔN) protein (A) Schematic flowchart of the protein purification process, including Ni-NTA affinity chromatography followed by HiTrap Heparin HP chromatography. (B) SDS-PAGE analysis of purified fractions during the HiTrap Heparin HP chromatography. The arrow indicates the target protein. M represents the molecular weight marker, with numbers on the right indicating molecular weights in kDa. The gel was stained with Coomassie Blue dye. (C) The HiTrap Heparin HP affinity chromatography profile of the final purified protein. An asterisk indicates the peak corresponding to the desired FOXP3.
Article Snippet: Alternatives: This protocol uses NEBNext Ultra II DNA Library Prep Kit with
Techniques: Expressing, Purification, Recombinant, Protein Purification, Affinity Chromatography, Chromatography, SDS Page, Molecular Weight, Marker, Staining